CALCRL anticorps (Cytoplasmic Domain)

N° du produit ABIN1048377

Cet anticorps anti-CALCRL est un anticorps Lapin Polyclonal détectant CALCRL dans IHC et IHC (p). Adapté pour Humain, Rat, Boeuf (Vache), Chien, Cheval, Lapin, Chévre, Singe, Hamster, Porc et Xenopus laevis. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour CALCRL anticorps (Cytoplasmic Domain) (ABIN1048377)

Antigène: CALCRL (Calcitonin Receptor-Like (CALCRL))

Reactivité: Humain, Rat, Boeuf (Vache), Chien, Cheval, Lapin, Chévre, Singe, Hamster, Porc, Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CALCRL est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-CALCRL anticorps

Épitope: Cytoplasmic Domain

Marque: IHC-plus™

Specificité: Human CALCRL. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except CALCR (56 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Goat, Hamster, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Xenopus (100%) Mouse, Elephant, Bat, Guinea pig, Stickleback, Pufferfish, Zebrafish (94%) Turkey, Chicken (81%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human CALCRL. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Goat, Hamster, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Xenopus (100%), Mouse, Elephant, Bat, Guinea pig, Stickleback, Pufferfish, Zebrafish (94%), Turkey, Chicken (81%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (34 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-CALCRL (ABIN1048377)

Zheng, Nixdorf-Bergweiler, van Brederode, Alzheimer, Messlinger: "Excitatory Effects of Calcitonin Gene-Related Peptide (CGRP) on Superficial Sp5C Neurons in Mouse Medullary Slices." dans: International journal of molecular sciences, Vol. 22, Issue 7, (2021) (PubMed).

Détails sur CALCRL

Antigène: CALCRL (Calcitonin Receptor-Like (CALCRL))

Autre désignation: CALCRL / CGRP Receptor

Sujet: Name/Gene ID: CALCRL
Subfamily: Adrenomedullin
Family: GPCR

Synonyms: CALCRL, CGRP type 1 receptor, Cgrp1 receptor, CRLR, Calcitonin receptor-like, CGRP receptor, CGRPR

ID gène: 10203

Pathways: cAMP Metabolic Process