Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

CCRL2 anticorps (Cytoplasmic Domain)

CCRL2 Reactivité: Humain IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1048410
  • Antigène Voir toutes CCRL2 Anticorps
    CCRL2 (Chemokine (C-C Motif) Receptor-Like 2 (CCRL2))
    Épitope
    • 9
    • 7
    • 7
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reactivité
    • 49
    • 8
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 42
    • 8
    • 3
    Lapin
    Clonalité
    • 43
    • 9
    • 1
    Polyclonal
    Conjugué
    • 31
    • 4
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CCRL2 est non-conjugé
    Application
    • 31
    • 25
    • 10
    • 6
    • 5
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human CCRL2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human (100%) Gibbon (95%) Gorilla (90%) Monkey (80%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 20 amino acid peptide from 2nd cytoplasmic domain of human CCRL2. Percent identity with other species by BLAST analysis: Human (100%), Gibbon (95%), Gorilla (90%), Monkey (80%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product CCRL2 Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (11 μg/mL)
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    CCRL2 (Chemokine (C-C Motif) Receptor-Like 2 (CCRL2))
    Autre désignation
    CCRL2 (CCRL2 Produits)
    Synonymes
    anticorps CCRL2, anticorps ACKR5, anticorps CKRX, anticorps CRAM, anticorps CRAM-A, anticorps CRAM-B, anticorps HCR, anticorps 1810047I05Rik, anticorps Ackr5, anticorps CCR11, anticorps Cmkbr1l2, anticorps E01, anticorps L-CCR, anticorps C-C motif chemokine receptor like 2, anticorps chemokine (C-C motif) receptor-like 2, anticorps Ccrl2, anticorps CCRL2
    Sujet
    Name/Gene ID: CCRL2
    Subfamily: Chemokine
    Family: GPCR

    Synonyms: CCRL2, CCR11, Chemokine receptor X, CRAM, CRAM-A, CRAM-B, CKRX, C-C chemokine receptor-like 2, Chemokine receptor CCR11, HCR
    ID gène
    9034
Vous êtes ici:
Support technique