G Protein-Coupled Receptor 115 anticorps (C-Term)

N° du produit ABIN1048693

Détail du produit anti-G Protein-Coupled Receptor 115 anticorps

Antigène: G Protein-Coupled Receptor 115 (GPR115)

Épitope: C-Term

Reactivité: Humain, Souris, Boeuf (Vache), Cheval, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp G Protein-Coupled Receptor 115 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Marque: IHC-plus™

Specificité: Human GPR115. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GPR113 (69 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Bovine, Horse (100%) Elephant, Dog, Rabbit (94%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 16 amino acid peptide from C-terminus of human GPR115. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Bovine, Horse (100%), Elephant, Dog, Rabbit (94%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (4 - 10 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur G Protein-Coupled Receptor 115

Antigène: G Protein-Coupled Receptor 115 (GPR115)

Autre désignation: ADGRF4 / GPR115 (GPR115 Produits)

Synonymes: anticorps PGR18, anticorps 4632435A09Rik, anticorps AV239010, anticorps GPR115, anticorps RGD1565216, anticorps adhesion G protein-coupled receptor F4, anticorps G protein-coupled receptor 115, anticorps ADGRF4, anticorps Adgrf4, anticorps GPR115

Sujet: Name/Gene ID: ADGRF4
Subfamily: Orphan-B
Family: GPCR

Synonyms: ADGRF4, GPR115, PGR18, G protein-coupled receptor 115, G-protein coupled receptor 115

ID gène: 221393