GPR3 anticorps (G Protein-Coupled Receptor 3) (Extracellular Domain)

Details for Product anti-GPR3 Antibody No. ABIN1048790
Antigène
  • GPR3
  • ACCA
  • Gpcr3
  • Gpcr20
  • Gpcr21
  • G protein-coupled receptor 3
  • G-protein coupled receptor 3
  • GPR3
  • Gpr3
Épitope
Extracellular Domain
18
8
7
6
5
4
3
2
2
2
1
1
Reactivité
Hamster, Humain, Singe, Souris, Rat (Rattus)
92
42
40
3
3
3
2
2
2
2
2
1
1
Hôte
Lapin
79
9
7
Clonalité
Polyclonal
Conjugué
Cet anticorp GPR3 est non-conjugé
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
45
40
30
25
13
4
2
1
1
1
1
1
Options
Marque IHC-plus™
Immunogène Synthetic 15 amino acid peptide from 2nd extracellular domain of human GPR3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster (100%), Rabbit (93%), Bovine, Panda, Pig (80%).

Type of Immunogen: Synthetic peptide
Specificité Human GPR3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster (100%) Rabbit (93%) Bovine, Panda, Pig (80%).
Purification Immunoaffinity purified
Plasmids, Primers & others Plasmids, Primers & others GPR3 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation GPR3 (GPR3 Antibody Extrait)
Sujet Name/Gene ID: GPR3
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR

Synonyms: GPR3, ACCA, ACCA orphan receptor, G protein-coupled receptor 3, GPCR3, Hacca, G-protein coupled receptor 3
ID gène 2827
Pathways cAMP Metabolic Process
Indications d'application Approved: ICC, IF, IHC, IHC-P (6 μg/mL)
Commentaires

Target Species of Antibody: Human

Procédure de l'essai

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C,-20 °C
Stockage commentaire Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Date de péremption 12 months
Images (Fournisseur)
Image no. 1 for anti-G Protein-Coupled Receptor 3 (GPR3) (Extracellular Domain) antibody (ABIN1048790) Anti-GPR3 antibody ABIN1048790 IHC staining of human brain, neurons and glia. Immuno...
Produit citée dans: Ledent, Demeestere, Blum, Petermans, Hämäläinen, Smits, Vassart: "Premature ovarian aging in mice deficient for Gpr3." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 25, pp. 8922-6, 2005 (PubMed).

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