GPR39 anticorps (Extracellular Domain)

N° du produit ABIN1048812

Cet anticorps anti-GPR39 est un anticorps Lapin Polyclonal détectant GPR39 dans IHC et IHC (p). Adapté pour Humain, Souris, Rat, Cheval et Lapin. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour GPR39 anticorps (Extracellular Domain) (ABIN1048812)

Antigène: GPR39 (G Protein-Coupled Receptor 39 (GPR39))

Reactivité: Humain, Souris, Rat, Cheval, Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GPR39 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GPR39 anticorps

Épitope: Extracellular Domain

Marque: IHC-plus™

Specificité: Human GPR39. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Horse, Rabbit (100%) Marmoset, Bovine, Panda, Turkey, Chicken (94%) Pig, Opossum (88%) Dog (81%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 16 amino acid peptide from 3rd extracellular domain of human GPR39. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Horse, Rabbit (100%), Marmoset, Bovine, Panda, Turkey, Chicken (94%), Pig, Opossum (88%), Dog (81%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (1.25 - 2.5 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-GPR39 (ABIN1048812)

Alkayed, Cao, Qian, Nagarajan, Liu, Nelson, Xie, Li, Fan, Liu, Grafe, Davis, Xiao, Barnes, Kaul: "Control of Coronary Vascular Resistance by Eicosanoids via a Novel GPCR." dans: , (2022) (PubMed).

Davis, Bah, Zhang, Nelson, Golgotiu, Nie, Alkayed, Young, Woltjer, Silbert, Grafe, Alkayed: "GPR39 localization in the aging human brain and correlation of expression and polymorphism with vascular cognitive impairment." dans: Alzheimer's & dementia (New York, N. Y.), Vol. 7, Issue 1, pp. e12214, (2021) (PubMed).

Détails sur GPR39

Antigène: GPR39 (G Protein-Coupled Receptor 39 (GPR39))

Autre désignation: GPR39

Sujet: Name/Gene ID: GPR39
Subfamily: Orphan-A
Family: GPCR

Synonyms: GPR39, G protein-coupled receptor 39, G-protein coupled receptor 39

ID gène: 2863

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Feeding Behaviour