Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

GPR75 anticorps (Cytoplasmic Domain)

GPR75 Reactivité: Humain, Cheval, Boeuf (Vache), Porc IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1048872
  • Antigène Voir toutes GPR75 Anticorps
    GPR75 (G Protein-Coupled Receptor 75 (GPR75))
    Épitope
    • 16
    • 15
    • 8
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reactivité
    • 52
    • 37
    • 14
    • 7
    • 7
    • 6
    • 6
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    Humain, Cheval, Boeuf (Vache), Porc
    Hôte
    • 66
    • 1
    Lapin
    Clonalité
    • 65
    • 1
    • 1
    Polyclonal
    Conjugué
    • 29
    • 7
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp GPR75 est non-conjugé
    Application
    • 46
    • 29
    • 14
    • 13
    • 13
    • 13
    • 11
    • 10
    • 8
    • 8
    • 3
    • 2
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human GPR75. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Elephant, Panda, Bovine, Horse, Pig, Opossum (100%) Gorilla, Monkey, Bat, Rabbit, Lizard, Xenopus, Pufferfish, Zebrafish (94%) Hamster (88%) Mouse, Rat, Chicken (81%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 16 amino acid peptide from 2nd cytoplasmic domain of human GPR75. Percent identity with other species by BLAST analysis: Human, Elephant, Panda, Bovine, Horse, Pig, Opossum (100%), Gorilla, Monkey, Bat, Rabbit, Lizard, Xenopus, Pufferfish, Zebrafish (94%), Hamster (88%), Mouse, Rat, Chicken (81%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product GPR75 Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (18 μg/mL)
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    GPR75 (G Protein-Coupled Receptor 75 (GPR75))
    Autre désignation
    GPR75 (GPR75 Produits)
    Synonymes
    anticorps GPR75, anticorps GPRchr2, anticorps WI31133, anticorps G protein-coupled receptor 75, anticorps G protein-coupled receptor 75 S homeolog, anticorps GPR75, anticorps gpr75, anticorps Gpr75, anticorps gpr75.S
    Sujet
    Name/Gene ID: GPR75
    Subfamily: Orphan-A
    Family: GPCR

    Synonyms: GPR75, G protein-coupled receptor 75, GPR-chr2, WI31133, GPRchr2, WI-31133
    ID gène
    10936
Vous êtes ici:
Support technique