Leukotriene B4 Receptor/BLT anticorps (Cytoplasmic Domain)

N° du produit ABIN1049000

L’anticorps Lapin Polyclonal anti-Leukotriene B4 Receptor/BLT a été validé pour IHC, IHC (fro) et IHC (p). Il convient pour détecter Leukotriene B4 Receptor/BLT dans des échantillons de Humain, Souris, Rat, Singe, Hamster, Cheval et Lapin. Il y a 1 publication disponible.

Aperçu rapide pour Leukotriene B4 Receptor/BLT anticorps (Cytoplasmic Domain) (ABIN1049000)

Antigène: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Reactivité: Humain, Souris, Rat, Singe, Hamster, Cheval, Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Leukotriene B4 Receptor/BLT est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Leukotriene B4 Receptor/BLT anticorps

Épitope: Cytoplasmic Domain

Marque: IHC-plus™

Specificité: Human Leukotriene B4 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Horse, Rabbit (100%) Bovine (88%) Opossum, Platypus (81%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human Leukotriene B4 Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Horse, Rabbit (100%), Bovine (88%), Opossum, Platypus (81%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-Fr, IHC-P (5 - 10 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-Leukotriene B4 Receptor/BLT (ABIN1049000)

Bäck, Bu, Bränström, Sheikine, Yan, Hansson: "Leukotriene B4 signaling through NF-kappaB-dependent BLT1 receptors on vascular smooth muscle cells in atherosclerosis and intimal hyperplasia." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 48, pp. 17501-6, (2005) (PubMed).

Détails sur Leukotriene B4 Receptor/BLT

Antigène: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Autre désignation: Leukotriene B4 Receptor / BLT1

Sujet: Name/Gene ID: LTB4R
Subfamily: Chemoattractant
Family: GPCR

Synonyms: LTB4R, BLT, BLT1, BLTR, Blt-1, Chemokine receptor-like 1, G protein-coupled receptor 16, GPR16, Leukotriene B4 receptor, Leukotriene B4 receptor 1, G-protein coupled receptor 16, LTBR1, p2Y purinoceptor 7, p2Y7, LTB4-R 1, p2RY7, CMKRL1, LTB4-R1, LTB4R1

ID gène: 1241