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LPAR4 anticorps (N-Term)

LPAR4 Reactivité: Humain, Souris, Rat, Singe, Hamster, Roussette (Chauve-souris) IHC, IHC (p), FACS Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1049014
  • Antigène Voir toutes LPAR4 Anticorps
    LPAR4 (Lysophosphatidic Acid Receptor 4 (LPAR4))
    Épitope
    • 15
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivité
    • 53
    • 30
    • 28
    • 9
    • 7
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    Humain, Souris, Rat, Singe, Hamster, Roussette (Chauve-souris)
    Hôte
    • 54
    Lapin
    Clonalité
    • 54
    Polyclonal
    Conjugué
    • 21
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp LPAR4 est non-conjugé
    Application
    • 42
    • 22
    • 13
    • 13
    • 10
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
    Marque
    IHC-plus™
    Specificité
    Human LPAR4 / GPR23. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bat, Hamster (100%) Dog, Bovine, Elephant, Panda, Rabbit, Horse, Pig (95%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 19 amino acid peptide from N-terminal extracellular domain of human LPAR4 / GPR23. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bat, Hamster (100%), Dog, Bovine, Elephant, Panda, Rabbit, Horse, Pig (95%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product LPAR4 Anticorps primaire
  • Indications d'application
    Approved: Flo, IHC, IHC-P (2 μg/mL)
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Lin, Boyce: "IL-4 regulates MEK expression required for lysophosphatidic acid-mediated chemokine generation by human mast cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 5430-8, (2005) (PubMed).

  • Antigène
    LPAR4 (Lysophosphatidic Acid Receptor 4 (LPAR4))
    Autre désignation
    LPAR4 / GPR23 (LPAR4 Produits)
    Synonymes
    anticorps GPR23, anticorps gpr23, anticorps lpa4, anticorps LPA4, anticorps P2RY9, anticorps P2Y5-LIKE, anticorps P2Y9, anticorps 5730485F04Rik, anticorps Gpr23, anticorps p2y9, anticorps RGD1563686, anticorps lysophosphatidic acid receptor 4, anticorps lysophosphatidic acid receptor 4 L homeolog, anticorps LPAR4, anticorps lpar4.L, anticorps lpar4, anticorps Lpar4
    Sujet
    Name/Gene ID: LPAR4
    Subfamily: Lysophospholipid/Lysosphingolipid
    Family: GPCR

    Synonyms: LPAR4, G-protein coupled receptor 23, LPA-4, p2RY9, p2Y5-LIKE, p2Y purinoceptor 9, p2Y5-like receptor, p2Y9, G protein-coupled receptor 23, GPR23, LPA receptor 4, LPA4, Purinergic receptor 9
    ID gène
    2846
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