PYGM anticorps (Internal Region)

N° du produit ABIN1049281

Cet anticorps Lapin Polyclonal détecte spécifiquement PYGM dans WB, IHC et IHC (p). Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Chien, Lapin, Mouton et Singe.

Aperçu rapide pour PYGM anticorps (Internal Region) (ABIN1049281)

Antigène: PYGM (Phosphorylase, Glycogen, Muscle (PYGM))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Lapin, Mouton, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PYGM est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PYGM anticorps

Épitope: Internal Region

Marque: IHC-plus™

Specificité: Human PYGM / Phosphorylase b. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Dog, Bovine, Elephant, Panda, Rabbit, Armadillo (100%) Shrew, Bat, Pig, Platypus (90%) Xenopus, Penicillium (80%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 10 amino acid peptide from internal region of human PYGM / Phosphorylase b. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Dog, Bovine, Elephant, Panda, Rabbit, Armadillo (100%), Shrew, Bat, Pig, Platypus (90%), Xenopus, Penicillium (80%).

Type of Immunogen: Synthetic phospho-peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL), WB

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur PYGM

Antigène: PYGM (Phosphorylase, Glycogen, Muscle (PYGM))

Autre désignation: PYGM

Sujet: Name/Gene ID: PYGM

Synonyms: PYGM, GPMM, Myophosphorylase, Phosphorylase, glycogen muscle

ID gène: 5837

Pathways: Cellular Glucan Metabolic Process