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PAR1 anticorps (N-Term)

F2R Reactivité: Humain, Singe IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1049406
  • Antigène Voir toutes PAR1 (F2R) Anticorps
    PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))
    Épitope
    • 16
    • 15
    • 8
    • 7
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivité
    • 69
    • 25
    • 23
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Humain, Singe
    Hôte
    • 62
    • 7
    • 2
    Lapin
    Clonalité
    • 65
    • 6
    Polyclonal
    Conjugué
    • 40
    • 7
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp PAR1 est non-conjugé
    Application
    • 61
    • 36
    • 13
    • 13
    • 11
    • 8
    • 8
    • 6
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human Thrombin Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GRIN1 (47 % ).
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gibbon, Baboon, Monkey (100%) Gorilla (95%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 19 amino acid peptide from N-terminal extracellular domain of human Thrombin Receptor. Percent identity with other species by BLAST analysis: Human, Gibbon, Baboon, Monkey (100%), Gorilla (95%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product F2R Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (7.2 μg/mL)
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))
    Autre désignation
    F2R / Thrombin Receptor / PAR1 (F2R Produits)
    Synonymes
    anticorps CF2R, anticorps HTR, anticorps PAR-1, anticorps PAR1, anticorps TR, anticorps Par1, anticorps TRGPC, anticorps AI482343, anticorps Cf2r, anticorps ThrR, anticorps Par-1, anticorps f2r-a, anticorps par1, anticorps coagulation factor II thrombin receptor, anticorps coagulation factor II (thrombin) receptor, anticorps coagulation factor 2 (thrombin) receptor L homeolog, anticorps F2R, anticorps F2r, anticorps f2r.L
    Sujet
    Name/Gene ID: F2R
    Subfamily: Proteinase-activated
    Family: GPCR

    Synonyms: F2R, CF2R, Coagulation factor II receptor, PAR1, PAR-1, Thrombin receptor, TR, HTR, Protease-activated receptor 1
    ID gène
    2149
    Pathways
    Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus
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