VIPR1 anticorps (Cytoplasmic Domain)

N° du produit ABIN1049455

Détail du produit anti-VIPR1 anticorps

Antigène: VIPR1 (Vasoactive Intestinal Peptide Receptor 1 (VIPR1))

Épitope: Cytoplasmic Domain

Reactivité: Humain, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp VIPR1 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human VIP Receptor 1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Elephant, Rabbit (88%) Mouse, Rat, Bovine, Guinea pig (81%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human VIP Receptor 1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Elephant, Rabbit (88%), Mouse, Rat, Bovine, Guinea pig (81%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur VIPR1

Antigène: VIPR1 (Vasoactive Intestinal Peptide Receptor 1 (VIPR1))

Autre désignation: VIPR1 (VIPR1 Produits)

Synonymes: anticorps HVR1, anticorps II, anticorps PACAP-R-2, anticorps PACAP-R2, anticorps RDC1, anticorps V1RG, anticorps VAPC1, anticorps VIP-R-1, anticorps VIPR, anticorps VIRG, anticorps VPAC1, anticorps VPAC1R, anticorps VPCAP1R, anticorps CVIPR, anticorps AV071699, anticorps VIP-R1, anticorps RATVASREC, anticorps VASREC, anticorps VIPR1, anticorps LOC100226500, anticorps vipr1, anticorps hvr1, anticorps rdc1, anticorps v1rg, anticorps vipr, anticorps virg, anticorps vapc1, anticorps vpac1, anticorps vpcap1, anticorps vpac1-r, anticorps VIP-R, anticorps vasoactive intestinal peptide receptor 1, anticorps vasoactive intestinal peptide receptor 1b, anticorps vasoactive intestinal peptide receptor 1 S homeolog, anticorps vasoactive intestinal polypeptide receptor, anticorps VIPR1, anticorps Vipr1, anticorps vipr1b, anticorps vipr1, anticorps vipr1.S, anticorps LOC100303683

Sujet: Name/Gene ID: VIPR1
Subfamily: Vasoactive intestinal polypeptide
Family: GPCR

Synonyms: VIPR1, HVR1, PACAP type II receptor, PACAP-R-2, Pvr2, RDC1, Vip receptor subtype 1, Vpac1 receptor, V1RG, VIP receptor 1, VIPR, VIRG, VPCAP1R, PACAP-R2, VAPC1, VIP and PACAP receptor 1, VPAC1R, Pacap receptor, type ii, Type II PACAP receptor, VIP receptor, type I, VIP-R-1, VIP1 receptor, VPAC1

ID gène: 7433