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WNK1 anticorps (C-Term)

WNK1 Reactivité: Humain, Souris, Rat, Singe, Lapin, Cheval, Hamster, Roussette (Chauve-souris) IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1049464
  • Antigène Voir toutes WNK1 Anticorps
    WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))
    Épitope
    • 15
    • 12
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 63
    • 37
    • 33
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Humain, Souris, Rat, Singe, Lapin, Cheval, Hamster, Roussette (Chauve-souris)
    Hôte
    • 78
    Lapin
    Clonalité
    • 69
    • 9
    Polyclonal
    Conjugué
    • 31
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp WNK1 est non-conjugé
    Application
    • 29
    • 27
    • 17
    • 13
    • 13
    • 12
    • 6
    • 5
    • 4
    • 3
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human WNK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Horse, Rabbit, Opossum (100%) Pig (94%) Bovine (88%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 17 amino acid peptide from near C-terminus of human WNK1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Horse, Rabbit, Opossum (100%), Pig (94%), Bovine (88%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product WNK1 Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (5 - 7.5 μg/mL)
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))
    Autre désignation
    WNK1 / p65 (WNK1 Produits)
    Synonymes
    anticorps HSAN2, anticorps HSN2, anticorps KDP, anticorps PRKWNK1, anticorps PSK, anticorps p65, anticorps WNK1, anticorps kdp, anticorps psk, anticorps hsn2, anticorps hsan2, anticorps pha2c, anticorps prkwnk1, anticorps 6430573H23Rik, anticorps EG406236, anticorps Hsn2, anticorps Prkwnk1, anticorps mKIAA0344, anticorps ATWNK1, anticorps T9J14.14, anticorps T9J14_14, anticorps ZIK4, anticorps with no lysine (K) kinase 1, anticorps WNK lysine deficient protein kinase 1, anticorps WNK lysine deficient protein kinase 1 L homeolog, anticorps serine/threonine-protein kinase WNK1, anticorps with no lysine (K) kinase 1, anticorps WNK1, anticorps wnk1.L, anticorps wnk1, anticorps LOC100056745, anticorps Wnk1
    Sujet
    Name/Gene ID: WNK1
    Subfamily: WNK
    Family: Protein Kinase

    Synonyms: WNK1, Erythrocyte 65 kDa protein, HSAN2, KIAA0344, Kinase deficient protein, HWNK1, PHA2C, PRKWNK1, PSK, HSN2, KDP
    ID gène
    65125
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