Papain anticorps

N° du produit ABIN105334

Détail du produit

Antigène: Papain

Reactivité: Papaya

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Réactivité croisée (Details): Cross reactivity against Papain from other sources may occur but has not been specifically determined.

Pureté: Anti-Papain antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Papain [Carica papaya].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Anti-Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya.
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Papain antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
ELISA Dilution: 1:10.000 - 1:50.000
Immunohistochemistry Dilution: 1:500 - 1:3.000
Western Blot Dilution: 1:1.000 - 1:5.000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: Expiration date is one (1) year from date of opening.

Détail du antigène

Antigène: Papain

Synonymes: anticorps PPI, anticorps Papain, anticorps papain-like, anticorps LOC110813108

Sujet: Papain Antibody detects Papain protein from papaya. Papain belongs to the peptidase C1 family and hydrolyses proteins with broad specificity for peptide bonds. The proteolysis preference of Papain is at amino acids bearing a large hydrophobic side chain at the P2 position and is inhibited when Val is at position P1. Papain may cause allergic reactions in humans. Anti-Papain Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research. Synonyms: Allergen Car p 1 antibody, Papaya proteinase I antibody, PPI antibody

UniProt: P00784