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Peroxidase anticorps

Cet anticorps anti-Peroxidase est un anticorps Cobaye Polyclonal détectant Peroxidase dans WB, ELISA et IHC. Adapté pour Raifort.
Rockland
N° du produit ABIN105506
N° du produit (Fournisseur): 200-2138-0100

Aperçu rapide pour Peroxidase anticorps (ABIN105506)

Antigène

Voir toutes Peroxidase Anticorps
Peroxidase

Reactivité

Raifort

Hôte

  • 4
  • 2
  • 1
  • 1
Cobaye

Clonalité

  • 7
  • 1
Polyclonal

Conjugué

  • 6
  • 2
Cet anticorp Peroxidase est non-conjugé

Application

  • 7
  • 6
  • 3
  • 3
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • N° du produit (Fournisseur)

    200-2138-0100

    Fournisseur

    Rockland

    Réactivité croisée (Details)

    Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.

    Pureté

    Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig Serum as well as purified and partially purified Peroxidase [Horseradish].

    niveau d'endotoxine

    Low Endotoxin : No

    Immunogène

    Peroxidase [Horseradish]
    Immunogen Type: Native Protein

    Isotype

    IgG
  • Indications d'application

    Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
    ELISA Dilution: 1:20.000 - 1:100.000
    Immunohistochemistry Dilution: 1:1.000 - 1:5.000
    Western Blot Dilution: 1:2.000 - 1:10.000

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Conseil sur la manipulation

    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

    Stock

    -20 °C

    Stockage commentaire

    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

    Date de péremption

    Expiration date is one (1) year from date of opening.
  • Antigène

    Peroxidase

    Sujet

    Horse Radish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is ideal for investigators in Microbiology, Immunology, and Cell Biology research. Synonyms: HRP, Horseradish peroxidase, Guinea pig-anti-peroxidase antibody
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