Peroxidase anticorps

N° du produit ABIN105506

Détail du produit anti-Peroxidase anticorps

Antigène: Peroxidase

Reactivité: Raifort

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp Peroxidase est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Réactivité croisée (Details): Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.

Pureté: Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig Serum as well as purified and partially purified Peroxidase [Horseradish].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Peroxidase [Horseradish]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
ELISA Dilution: 1:20.000 - 1:100.000
Immunohistochemistry Dilution: 1:1.000 - 1:5.000
Western Blot Dilution: 1:2.000 - 1:10.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur Peroxidase

Antigène: Peroxidase

Abstract: Peroxidase Produits

Synonymes: anticorps peroxidase, anticorps PER1, anticorps ShK domain and PerOxidase domain containing protein, anticorps Peroxidase skpo-1, anticorps hypothetical protein, anticorps peroxidase 1, anticorps 1-Cys-peroxiredoxine, anticorps skpo-3, anticorps skpo-1, anticorps T06D8.10, anticorps C46A5.4, anticorps K10B4.1, anticorps R08F11.7, anticorps WSP1, anticorps PER1

Sujet: Horse Radish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is ideal for investigators in Microbiology, Immunology, and Cell Biology research. Synonyms: HRP, Horseradish peroxidase, Guinea pig-anti-peroxidase antibody