Phosphothreonine anticorps

N° du produit ABIN105601

N° du produit (Fournisseur): 500-301-263

Cet anticorps Souris Monoclonal détecte spécifiquement Phosphothreonine dans WB et ELISA. Il présente une réactivité envers .

Aperçu rapide pour Phosphothreonine anticorps (ABIN105601)

Antigène: Phosphothreonine

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Phosphothreonine est non-conjugé

Application: Western Blotting (WB), ELISA

Clone: 18F6

Détail du produit anti-Phosphothreonine anticorps

N° du produit (Fournisseur): 500-301-263

Fournisseur: Rockland

Fonction: Phosphothreonine Antibody

Réactivité croisée (Details): Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphoserine or phosphotyrosine.

Attributs du produit: Synonyms: mouse anti-Phosphothreonine Antibody, mouse anti-pT antibody

Purification: This product is clarified ascites produced in balb/c mice using clone 18F6.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Phosphothreonine antibody was produced after repeated immunizations of balb/c mice with phosphothreonine conjugated KLH.

Immunogen Type: Other

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Application Note: This monoclonal antibody has been tested in ELISA and Western Blot and reacts specifically with phosphothreonine and shows minimal reactivity by ELISA, and competitive ELISA, with phosphoserine or phosphotyrosine.  The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, as well as phosphothreonine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation.  Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases and is important for cell signaling in activation, proliferation and differentiation.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Phosphothreonine

Antigène: Phosphothreonine

Classe de substances: Amino Acid

Sujet: Background: Phospho-Threonine Antibody detects proteins and peptides phosphorylated at threonine residues in a manner largely independent of the surrounding amino acid sequence. Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.