RFX5 anticorps (N-Term)

N° du produit ABIN106546

N° du produit (Fournisseur): 200-401-193

Cet anticorps Lapin Polyclonal détecte spécifiquement RFX5 dans WB, ELISA et GS. Il présente une réactivité envers Humain.

Aperçu rapide pour RFX5 anticorps (N-Term) (ABIN106546)

Antigène: RFX5 (Regulatory Factor X 5 (RFX5))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RFX5 est non-conjugé

Application: Western Blotting (WB), ELISA, Gel Shift (GS)

Détail du produit anti-RFX5 anticorps

Épitope: N-Term

N° du produit (Fournisseur): 200-401-193

Fournisseur: Rockland

Fonction: RFX5 Antibody

Réactivité croisée (Details): Anti-RFX5 (N-terminal specific) may react with unknown minor bands at 55 kDa and 85 kDa.

Attributs du produit: Synonyms: rabbit anti-RFX5 antibody, DNA binding protein RFX 5 antibody, Influences HLA class II expression antibody, Regulatory factor X 5 antibody, Regulatory factor X subunit 5 antibody, Regulatory factor X 5 (influences HLA class II expression) antibody

Purification: This product was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: RFX5 (N-terminal specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Gel Shift Dilution: 0.5 to 1.0 μL

Application Note: This product was assayed by immunoblot and found to be reactive against RFX5 (N-terminal specific) from a variety of fibroblast and B-cell lysates at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302.  Anti-RFX5 (N-terminal specific) is suitable for the detection by immunoblot of human RFX5 a shows a 75 kDa band.  This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 μL per assay.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur RFX5

Antigène: RFX5 (Regulatory Factor X 5 (RFX5))

Autre désignation: RFX5

Sujet: Background: DNA-binding protein RFX5 activates transcription from class II MHC promoters and recognizes X-boxes. It mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. RFX5 may be associated with Bare Lymphocyte Syndrome Type li Complementation group C and Mhc Class Ii Deficiency. Anti-RFX5 Antibody is useful for researchers interested in Transcription factors, Tuberculosis, and Allograft rejection.

ID gène: 5993

UniProt: P48382