Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
Specificité
TGM7 antibody is predicted to not cross-react with other TGase protein family members. At least two isoforms of TGM7 are known to exist, this antibody will recognize both isoforms.
Purification
Affinity chromatography purified via peptide column
Immunogène
TGM7 antibody was raised against a AA 13 synthetic peptide near the center of human TGM7.
TGM7 antibody can be used for detection of TGM7 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL. Optimal dilutions for each application to be determined by the researcher.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
PBS containing 0.02 % sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Stock
4 °C/-20 °C
Stockage commentaire
TGM7 antibody can be stored at 4° C for three months and -20° C, stable for up to one year.
Transglutaminases (TGM) are a family of structurally and functionally related Ca2+-dependent enzymes (TGases) that stabilize protein assemblies through the formation of gamma-glutamyl-epsilon lysine crosslinks. TGases influence numerous biological processes, including blood coagulation, cell differentiation, fertilization and apoptosis. TGM7 belongs to the transglutaminase superfamily and is also thought to catalyze the cross-linking of proteins, often resulting in stabilization of protein assemblies. Little is known of the role of TGM7, but defects in the highly related protein TGM5 are associated with acral peeling skin syndrome.