Actin, gamma 1 anticorps (AA 1-375)

N° du produit ABIN1077746

Détail du produit anti-Actin, gamma 1 anticorps

Antigène: Actin, gamma 1 (ACTG1)

Épitope: AA 1-375

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Actin, gamma 1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Séquence: MGHHHHHHSG SEF-MEEEIAALVI DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS TFQQMWISKQ EYDESGPSIV HRKCF

Specificité: It has been selected for its ability to recognize ACTg1 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogène: Recombinant ACTg1 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against ACTg1.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Commentaires:

Content: The quality control contains recombinant ACTg1 (Met1~Phe375) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Date de péremption: 12 months

Détails sur Actin, gamma 1

Antigène: Actin, gamma 1 (ACTG1)

Autre désignation: Actin Gamma 1 (ACTg1) (ACTG1 Produits)

Synonymes: anticorps ACT, anticorps ACTG, anticorps BRWS2, anticorps DFNA20, anticorps DFNA26, anticorps ACTG1, anticorps ACTA1, anticorps actg1a, anticorps Actg, anticorps actg1b, anticorps AL023024, anticorps Actl, anticorps E51, anticorps actin, anticorps cytoplasmic, anticorps actin gamma 1, anticorps actin, gamma 1 a, anticorps actin, gamma 1, anticorps actin gamma 1 S homeolog, anticorps actin, gamma, cytoplasmic 1, anticorps Actin, gamma 1, anticorps ACTG1, anticorps actg1, anticorps Actg1, anticorps actg1.S

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization