Calcitonin anticorps (AA 28-138)

N° du produit ABIN1077887

Détail du produit anti-Calcitonin anticorps

Antigène: Calcitonin (Calca)

Épitope: AA 28-138

Reactivité: Poulet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Calcitonin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Séquence: MGHHHHHHSG SEF- VRP GLESITDRVT LSDYEARRLL NALVKDFIQM TAEELEQASE GNSLDRPISK RCASLSTCVL GKLSQELHKL QTYPRTDVGA GTPGKKRNVL NDLDHERYAN YGETLGNN

Specificité: It has been selected for its ability to recognize CT in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogène: Recombinant CT expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against CT.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Commentaires:

Content: The quality control contains recombinant CT (Val28~Asn138) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Date de péremption: 12 months

Détails sur Calcitonin

Antigène: Calcitonin (Calca)

Autre désignation: Calcitonin (CT) (Calca Produits)

Synonymes: anticorps calcitonin-related polypeptide alpha, anticorps CALCA