A2BP1 anticorps (AA 394-646)

N° du produit ABIN108467

L’anticorps Lapin Polyclonal anti-A2BP1 a été validé pour WB et IF. Il convient pour détecter A2BP1 dans des échantillons de Chlamydomonas reinhardtii. Il y a 1 publication disponible.

Aperçu rapide pour A2BP1 anticorps (AA 394-646) (ABIN108467)

Antigène: A2BP1 (Ataxin 2-Binding Protein 1 (A2BP1))

Reactivité: Chlamydomonas reinhardtii

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp A2BP1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-A2BP1 anticorps

Épitope: AA 394-646

Réactivité croisée (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Homologie: Chlamydomonas reinhardtii

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 122 / 120 kDa

Purification: serum

Immunogène: residues 394-646 from Chlamydomonas reinhardtii FOX1 A8IZT9 fused to TrxA

Information d'application

Indications d'application: 1: 1000 - 1: 5000 with standard ECL (WB),

Commentaires:

This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water.

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Références pour anticorps anti-A2BP1 (ABIN108467)

Terauchi, Peers, Kobayashi, Niyogi, Merchant: "Trophic status of Chlamydomonas reinhardtii influences the impact of iron deficiency on photosynthesis." dans: Photosynthesis research, Vol. 105, Issue 1, pp. 39-49, (2010) (PubMed).

Détails sur A2BP1

Antigène: A2BP1 (Ataxin 2-Binding Protein 1 (A2BP1))

Autre désignation: FOX1

Sujet: A copper-containing protein in Chlamydomonas reinhardtii is a plasma membrane ferroxidase, encoded by the FOX1 gene, involved in Fe assimilation. FOX1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin, its occurrence and pattern of expression in Chlamydomonas indicate a role for copper in iron assimilation in a photosynthetic species.

Poids moléculaire: expected: 122 kDa, apparent: 120 kDa

ID gène: 394646

UniProt: A8IZT9