A2BP1 anticorps (AA 394-646)

N° du produit ABIN108467

Il existe 1 publication pour ce produit. L’anticorps anti-A2BP1 Polyclonal Lapin est utilisé pour la détection de A2BP1 dans des échantillons de Chlamydomonas reinhardtii. Il a été validé pour WB et IF.

Aperçu rapide pour A2BP1 anticorps (AA 394-646) (ABIN108467)

Antigène: A2BP1 (Ataxin 2-Binding Protein 1 (A2BP1))

Reactivité: Chlamydomonas reinhardtii

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp A2BP1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-A2BP1 anticorps

Épitope: AA 394-646

Fonction: Anti-FOX1 | plasma membrane ferroxidase FLP1

Réactivité croisée (Details): Chlamydomonas reinhardtii
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Chlamydomonas reinhardtii

Purification: Serum

Immunogène: residues 394-646 from Chlamydomonas reinhardtii FOX1 A8IZT9 fused to TrxA

Information d'application

Indications d'application: 1 : 1000-1 : 5000 (WB)

Commentaires:

This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Buffer: Serum

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Références pour anticorps anti-A2BP1 (ABIN108467)

Terauchi, Peers, Kobayashi, Niyogi, Merchant: "Trophic status of Chlamydomonas reinhardtii influences the impact of iron deficiency on photosynthesis." dans: Photosynthesis research, Vol. 105, Issue 1, pp. 39-49, (2010) (PubMed).

Détails sur A2BP1

Antigène: A2BP1 (Ataxin 2-Binding Protein 1 (A2BP1))

Autre désignation: FOX1

Sujet:

Background: A copper-containing protein in Chlamydomonas reinhardtii is a plasma membrane ferroxidase, encoded by the FOX1 gene, involved in Fe assimilation. FOX1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin, its occurrence and pattern of expression in Chlamydomonas indicate a role for copper in iron assimilation in a photosynthetic species.

Additional information: FOX1 is 10-20-fold induced in iron-deficiency and its occurrence therefore serves as a marker of iron nutrition state in Chlamydomonas. For detection image please refer to the article below.

Poids moléculaire: 122 | 120 kDa

ID gène: 394646

UniProt: A8IZT9