A2BP1 anticorps (AA 394-646)

N° du produit ABIN108467

Détail du produit anti-A2BP1 anticorps

Antigène: A2BP1 (Ataxin 2-Binding Protein 1 (A2BP1))

Épitope: AA 394-646

Reactivité: Chlamydomonas reinhardtii

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp A2BP1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Homologie: Chlamydomonas reinhardtii

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 122 / 120 kDa

Purification: serum

Immunogène: residues 394-646 from Chlamydomonas reinhardtii FOX1 A8IZT9 fused to TrxA

Information d'application

Indications d'application: 1: 1000 - 1: 5000 with standard ECL (WB),

Commentaires:

This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water.

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Références pour anticorps anti-A2BP1 (ABIN108467)

Terauchi, Peers, Kobayashi, Niyogi, Merchant: "Trophic status of Chlamydomonas reinhardtii influences the impact of iron deficiency on photosynthesis." dans: Photosynthesis research, Vol. 105, Issue 1, pp. 39-49, (2010) (PubMed).

Détails sur A2BP1

Antigène: A2BP1 (Ataxin 2-Binding Protein 1 (A2BP1))

Autre désignation: FOX1 (A2BP1 Produits)

Synonymes: anticorps A2BP1, anticorps fox1, anticorps a2bp1, anticorps zgc:103635, anticorps 2BP1, anticorps FOX-1, anticorps FOX1, anticorps HRNBP1, anticorps A2bp, anticorps A2bp1, anticorps Hrnbp1, anticorps fox-1, anticorps RNA binding protein, fox-1 homolog 1, anticorps RNA binding fox-1 homolog 1, anticorps multicopper ferroxidase, anticorps RNA binding protein, fox-1 homolog (C. elegans) 1, anticorps RBFOX1, anticorps rbfox1, anticorps FOX1, anticorps Rbfox1

Sujet: A copper-containing protein in Chlamydomonas reinhardtii is a plasma membrane ferroxidase, encoded by the FOX1 gene, involved in Fe assimilation. FOX1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin, its occurrence and pattern of expression in Chlamydomonas indicate a role for copper in iron assimilation in a photosynthetic species.

Poids moléculaire: expected: 122 kDa, apparent: 120 kDa

ID gène: 394646

UniProt: A8IZT9