Phosphoglucomutase 1 anticorps (Biotin)

N° du produit ABIN1099701

Détail du produit anti-Phosphoglucomutase 1 anticorps (Biotin)

Antigène: Phosphoglucomutase 1 (PGM1)

Reactivité: Lapin

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp Phosphoglucomutase 1 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Dot Blot (DB)

Specificité: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Attributs du produit: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Physicochemical characteristics: IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 6.8. No foreign proteins added. Marker N-Hydroxysuccinimidobiotin.

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogène: Phosphoglucomutase isolated and purified from rabbit muscle. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/20 and 1/500.

Commentaires:

Physical form: Biotin-coupled hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Conjugation procedure: A proprietary technique for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilised to assure stability and long shelf life.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Phosphate buffered saline (PBS, pH 7.2). No foreign protein added.

Agent conservateur: Without preservative

Conseil sur la manipulation: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Stock: 4 °C

Détails sur Phosphoglucomutase 1

Antigène: Phosphoglucomutase 1 (PGM1)

Autre désignation: phosphoglucomutase (PGM1 Produits)

Synonymes: anticorps CDG1T, anticorps GSD14, anticorps ARABIDOPSIS THALIANA PHOSPHOGLUCOMUTASE, anticorps ATPGMP, anticorps MIO24.4, anticorps MIO24_4, anticorps PGM1, anticorps PHOSPHOGLUCOMUTASE, anticorps STARCH-FREE 1, anticorps STF1, anticorps phosphoglucomutase, anticorps PSPTO3035, anticorps CMS0426, anticorps 3230402E02Rik, anticorps Pgm-1, anticorps Pgm2, anticorps zgc:63718, anticorps phosphoglucomutase-1, anticorps pgm2, anticorps PGM, anticorps PGM 1, anticorps phosphoglucomutase 1, anticorps hypothetical protein, anticorps phosphoglucomutase, anticorps phosphoglucomutase alpha-D-glucose phosphate-specific Pgm, anticorps phosphoglucomutase, alpha-D-glucose phosphate-specific, anticorps phosphoglucomutase 1 L homeolog, anticorps PGM1, anticorps R05F9.6, anticorps PGM, anticorps pgm, anticorps STY0736, anticorps PMI_RS02700, anticorps Pgm1, anticorps pgm1, anticorps LOC542721, anticorps pgm1.L

Sujet: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Pathways: Cellular Glucan Metabolic Process