3-Phosphoglyceric Phosphokinase anticorps (Biotin)

N° du produit ABIN1099703

L’anticorps anti-3-Phosphoglyceric Phosphokinase Polyclonal Lapin est utilisé pour la détection de 3-Phosphoglyceric Phosphokinase dans des échantillons de Saccharomyces cerevisiae. Il a été validé pour ELISA, WB, DB, ICC et IHC (p).

Aperçu rapide pour 3-Phosphoglyceric Phosphokinase anticorps (Biotin) (ABIN1099703)

Antigène: 3-Phosphoglyceric Phosphokinase

Reactivité: Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Biotin

Application: ELISA, Western Blotting (WB), Dot Blot (DB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit

Fonction: Rabbit anti Saccharomyces cerevisiae 3-Phosphoglyceric phosphokinase, conjugated with Biotin

Specificité: Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to 3-phosphoglyceric phosphokinase from baker's yeast.

Réactivité croisée (Details): Cross-reactivities against enzymes of other sources may occur but have not been determined.

Attributs du produit: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Purification: Purified IgG fraction from antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogène: 3-Phosphoglyceric phosphokinase isolated and purified from baker's yeast. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Détail du antigène

Antigène: 3-Phosphoglyceric Phosphokinase