CKB anticorps (N-Term)

N° du produit ABIN1106703

Cet anticorps anti-CKB est un anticorps Souris Monoclonal détectant CKB dans WB, IF, IHC (fro) et IHC (p). Adapté pour Humain, Souris, Rat et Lapin.

Aperçu rapide pour CKB anticorps (N-Term) (ABIN1106703)

Antigène: CKB (Creatine Kinase, Brain (CKB))

Reactivité: Humain, Souris, Rat, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CKB est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CK-BYK-21E10

Détail du produit anti-CKB anticorps

Épitope: N-Term

Séquence: SNSHNALKLR FPAEDEF

Specificité: The monoclonal antibody CK-BYK/21E10 recognizes creatine kinase B-type, also known as CKB.

Réactivité croisée (Details): Species reactivity (tested):Human, mouse, rat, rabbit

Purification: Protein G

Immunogène: Synthetic 17-mer peptide corresponding to the N-terminal sequence of human creatine kinase brain-type

Isotype: IgG2b

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2 - 8 °C.

Détails sur CKB

Antigène: CKB (Creatine Kinase, Brain (CKB))

Autre désignation: CKBB

Sujet: Human CKB is a protein of 381 amino acids (~45 kDa), expressed in a number of tissues. CKB is most abundant in adult brain, approx. 5-fold lower in the stomach, 10-fold lower in the heart and barely detectable in liver. In brain, whereas most CKB has been shown to be cytosolic, several of the reactions requiring CKB are membrane-associated. CKB belongs to the creatine kinase (CK) isoenzymes that catalyse the synthesis of phosphocreatine (PCr) and its subsequent use in the regeneration of ATP in cell types where the consumption of ATP is rapid and/or sudden. In the brain the different CK isoforms constitute an energy shuttle wherein ATP produced in the mitochondria is used by a mitochondrial CK [e.g. ubiquitous mitochondrial creatine kinase (uMi-CK)] to generate PCr, which is then transported and used by a cytoplasmic CK [e.g. brain creatine kinase (CKB)] to regenerate ATP at discrete cellular sites of high ATP turnover. CKB appears to have a role in regenerating ATP needed for the transport of ions and neurotransmitters since CKB has been localized to brain synaptic plasma membranes, possibly coupled to Na+/K+-ATPase and acetylcholine receptor-rich membranes. Expression of CKB is developmentally controlled: in rat, brain CKB protein at birth is extremely low and increases 10-fold until week 4. This reflects the many energy-demanding processes in brain during brain development.Synonyms: B-CK, CKB, Creatine kinase B chain, Creatine kinase B-type, Creatine kinase BB

ID gène: 1152

NCBI Accession: NP_001814

UniProt: P12277