Complex IV Subunit I (AA 2-14), (N-Term) anticorps

N° du produit ABIN1106818

Cet anticorps Lapin Polyclonal détecte spécifiquement dans WB. Il présente une réactivité envers Humain.

Aperçu rapide pour Complex IV Subunit I (AA 2-14), (N-Term) anticorps (ABIN1106818)

Antigène: Complex IV Subunit I

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB)

Détail du produit

Épitope: AA 2-14, N-Term

Specificité: This antibody detects Complex IV subunit I (N-term).

Réactivité croisée (Details): Species reactivity (tested):Human

Purification: Immunogen affinity purified

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminal of human CO1 (2-14 aa, FADRWLFSTNHKD) .Remarks: Sequence is identical to the related rat and mouse sequence.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Reconstitution: 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/ml.

Buffer: 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Détail du antigène

Antigène: Complex IV Subunit I

Sujet: Cytochrome c oxidase subunit I (CO1 or MTCO1) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb,VIIc, and VIII are nuclear encoded.1,2 The cytochrome c oxidase family of enzymes have 4 redox centers ,2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I.3 Acin-Perez et al. (2003)identified a cell line containing single and double missense mutations in the cytochrome c oxidase (COX)subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant, cultured cells can maintain several mtDNA haplotypes at stable frequencies, the respiratory chain has little spare COX capacity, and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.4Synonyms: COI, COXI, Cytochrome c oxidase subunit 1, MT-CO1, MTCO1, Mitochondria Complex IV (Cytochrome C Oxidase)

ID gène: 4512

NCBI Accession: AP_000641

UniProt: P00395