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eEF1A1 anticorps (N-Term)

L’anticorps Souris Monoclonal anti-eEF1A1 a été validé pour WB, IF et EIA. Il convient pour détecter eEF1A1 dans des échantillons de Bactéries.
N° du produit ABIN1107053

Aperçu rapide pour eEF1A1 anticorps (N-Term) (ABIN1107053)

Antigène

Voir toutes eEF1A1 (EEF1A1) Anticorps
eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Reactivité

  • 72
  • 39
  • 39
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Bactéries

Hôte

  • 66
  • 4
  • 2
Souris

Clonalité

  • 59
  • 13
Monoclonal

Conjugué

  • 35
  • 5
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp eEF1A1 est non-conjugé

Application

  • 47
  • 20
  • 17
  • 15
  • 14
  • 14
  • 11
  • 10
  • 8
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone

9E2
  • Épitope

    • 15
    • 12
    • 8
    • 7
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    Specificité

    The monoclonal antibody 900 recognizes elongation factor Tu (EF-Tu), a 43 kDa multifunctional protein present in Escherichia coli. The antibody recognizes EF-Tu in organisms belonging to bacterial and archaeal domains, yet no organisms from the eukaryotic domain. The panbacterial distribution of EF-Tu, which is present in large amounts in every prokaryotic cell, renders this protein a good candidate for diagnostic purposes. The highly conserved epitope recognized by monoclonal antibody 900 is located at the very end of the N-terminus of the EF-Tu molecule (SKEKFE).

    Réactivité croisée (Details)

    Species reactivity (tested):E. coli. Cross react with Bacteroides fragilis, Streptococcus oralis, Bacillus subtilis, Pseudomonas aeruginosa, Burkholderia cepacia, and Deinococcus sp., weakly cross reacts with Mycobacterium tuberculosis.

    Purification

    Protein G purified

    Immunogène

    E. coli K-12 C600 Rif ( pKT146) bacteria

    Isotype

    IgG2a
  • Indications d'application

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Concentration

    0.1 mg/mL

    Buffer

    PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C

    Stockage commentaire

    Product should be stored at 2-8 °C.
  • Antigène

    eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

    Autre désignation

    EEF1A1 / LENG7

    Sujet

    EF-Tu is one of the most abundant proteins present in prokaryotes, representing about 5 % of the total cellular protein of E. coli. During protein biosynthesis, the elongation process, EF-Tu catalyzes the binding of each aminoacyl-tRNA to the ribosome. It also interacts with several macromolecules and guanine nucleotides, including EF-Ts, GDP, GTP, and some ribosomal proteins.Synonyms: CCS-3, CCS3, EEF1A, EF-1-alpha-1, EF-Tu, EF1A, Elongation factor 1 alpha 1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, FLJ25721, GRAF-1EF, Leukocyte receptor cluster member 7, PTI1, eEF1A-1

    ID gène

    1915

    NCBI Accession

    NP_001393

    UniProt

    P68104
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