eEF1A1 anticorps (N-Term)

N° du produit ABIN1107053

L’anticorps anti-eEF1A1 Monoclonal Souris est utilisé pour la détection de eEF1A1 dans des échantillons de Bactéries. Il a été validé pour WB, IF et EIA.

Aperçu rapide pour eEF1A1 anticorps (N-Term) (ABIN1107053)

Antigène: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Reactivité: Bactéries

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp eEF1A1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: 9E2

Détail du produit anti-eEF1A1 anticorps

Épitope: N-Term

Specificité: The monoclonal antibody 900 recognizes elongation factor Tu (EF-Tu), a 43 kDa multifunctional protein present in Escherichia coli. The antibody recognizes EF-Tu in organisms belonging to bacterial and archaeal domains, yet no organisms from the eukaryotic domain. The panbacterial distribution of EF-Tu, which is present in large amounts in every prokaryotic cell, renders this protein a good candidate for diagnostic purposes. The highly conserved epitope recognized by monoclonal antibody 900 is located at the very end of the N-terminus of the EF-Tu molecule (SKEKFE).

Réactivité croisée (Details): Species reactivity (tested):E. coli. Cross react with Bacteroides fragilis, Streptococcus oralis, Bacillus subtilis, Pseudomonas aeruginosa, Burkholderia cepacia, and Deinococcus sp., weakly cross reacts with Mycobacterium tuberculosis.

Purification: Protein G purified

Immunogène: E. coli K-12 C600 Rif ( pKT146) bacteria

Isotype: IgG2a

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 2-8 °C.

Détails sur eEF1A1

Antigène: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Autre désignation: EEF1A1 / LENG7

Sujet: EF-Tu is one of the most abundant proteins present in prokaryotes, representing about 5 % of the total cellular protein of E. coli. During protein biosynthesis, the elongation process, EF-Tu catalyzes the binding of each aminoacyl-tRNA to the ribosome. It also interacts with several macromolecules and guanine nucleotides, including EF-Ts, GDP, GTP, and some ribosomal proteins.Synonyms: CCS-3, CCS3, EEF1A, EF-1-alpha-1, EF-Tu, EF1A, Elongation factor 1 alpha 1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, FLJ25721, GRAF-1EF, Leukocyte receptor cluster member 7, PTI1, eEF1A-1

ID gène: 1915

NCBI Accession: NP_001393

UniProt: P68104