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GFP anticorps

Cet anticorps anti-GFP est un anticorps Souris Monoclonal détectant GFP dans WB, IF, EIA et IHC (p). Adapté pour Aequorea victoria.
N° du produit ABIN1107357

Aperçu rapide pour GFP anticorps (ABIN1107357)

Antigène

Voir toutes GFP Anticorps
GFP (Green Fluorescent Protein (GFP))

Reactivité

  • 180
  • 15
  • 11
  • 9
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  • 3
  • 3
  • 3
Aequorea victoria

Hôte

  • 74
  • 64
  • 34
  • 14
  • 9
  • 5
  • 5
  • 4
  • 1
Souris

Clonalité

  • 115
  • 91
Monoclonal

Conjugué

  • 90
  • 13
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  • 4
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  • 3
  • 3
  • 2
  • 2
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  • 2
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  • 1
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Cet anticorp GFP est non-conjugé

Application

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  • 1
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Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

4B10B2
  • Réactivité croisée (Details)

    Species reactivity (tested):Human.

    Purification

    Purified

    Isotype

    IgG2a
  • Indications d'application

    ELISA: 1/10000.
    Western Bloting: 1/500 - 1/2000.
    Flow Cytometry: 1/200 - 1/400.
    Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.

    Restrictions

    For Research Use only
  • Buffer

    PBS, 0.03 % Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Antigène

    GFP (Green Fluorescent Protein (GFP))

    Autre désignation

    GFP

    Sujet

    GFP (Green Fluorescence Protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.Synonyms: GFP-Tag, Green fluorescent protein

    Poids moléculaire

    27 kDa
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