Nitrotyrosine anticorps

N° du produit ABIN1108460

L’anticorps Souris Monoclonal anti-Nitrotyrosine a été validé pour WB, IHC (fro), IHC (p) et EIA. Il convient pour détecter Nitrotyrosine dans des échantillons de .

Aperçu rapide pour Nitrotyrosine anticorps (ABIN1108460)

Antigène: Nitrotyrosine

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nitrotyrosine est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: HM-11

Détail du produit anti-Nitrotyrosine anticorps

Purification: Protein G

Immunogène: Nitrated KLH

Isotype: IgG2b

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % bovine serum albumin

Conseil sur la manipulation: Keep Sterile, Do Not Add Sodium Azide or any Nitric containing preservative since this will affect the antibody!

Stock: 4 °C

Stockage commentaire: Store at 2 - 8 °C.

Détails sur Nitrotyrosine

Antigène: Nitrotyrosine

Classe de substances: Chemical

Sujet: Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Inflammation is characterized by increased nitric oxide (NO) production. NO reacts rapidly with superoxide to form peroxynitrite. At physiological? pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, Amyotrophic Lateral Sclerosis (ALS) and Multiple Sclerosis (MS). Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo and consequently as a marker of NO-mediated tissue damage.Synonyms: NO-Tyrosine, Nitro-Tyrosine