PRTN3 anticorps

N° du produit ABIN1108720

Cet anticorps Souris Monoclonal détecte spécifiquement PRTN3 dans WB, IHC (fro), EIA, IHC (p) et Func. Il présente une réactivité envers Humain.

Aperçu rapide pour PRTN3 anticorps (ABIN1108720)

Antigène: PRTN3 (Proteinase 3 (PRTN3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PRTN3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Clone: WGM2

Détail du produit anti-PRTN3 anticorps

Specificité: Monoclonal antibody WGM2 reacts with human proteinase 3 (PR3), a 30 kDa protein.

Réactivité croisée (Details): Species reactivity (tested):Human

Purification: Protein G

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % bovine serum albumin

Stock: 4 °C

Stockage commentaire: Store at 2 - 8 °C.

Détails sur PRTN3

Antigène: PRTN3 (Proteinase 3 (PRTN3))

Autre désignation: PR3 / C-ANCA

Sujet: PR3 is a major antigen recognized by autoantibodies directed against cytoplasmic proteins of neutrophilic granulocytes and monocytes (so called anti-neutrophil cytoplasmic autoantibodies (ANCA)). ANCA are able to activate primed neutrophils to produce oxygen radicals and release lytic enzymes, including PR3. Proteinase 3 (PR3) was identified as the target antigen of ANCA in Wegener's granulomatosis (WG). ANCA directed against PR3 (PR3-ANCA) can interfere with the binding of PR3 to its physiological inhibitor alpha1-antitrypsin (alpha1-AT) and with the proteolytic activity of PR3. At the site of inflammation PR3 can cleave the complex between these inhibiting ANCA and PR3 itself, leaving active PR3. Autoantibodies to PR3 are potent activators of the 5-lipoxygenase pathway in primed human neutrophils. Extracellular free arachidonic acid, as present at an inflammatory focus, synergizes with such autoantibodies to evoke full-blown lipid mediator generation, granule secretion and respiratory burst. Proteinase 3 (PR3) is a neutral serine proteinase, which is localized in the azurophilic granules of neutrophils and in granules of monocytes and can be detected in the membrane of secretory vesicles. PR3 degrades a number of extracellular matrix proteins such as elastin and inactivates human C1 inhibitor. Membrane-associated PR3 is also able to activate caspase-3 without triggering apoptosis of neutrophils, which is possibly a neutrophil survival mechanism. In addition, PR3 is involved in myeloid differentiation and is, therefore, also called myeloblastin.Synonyms: AGP7, Leukocyte proteinase 3, MBN, Myeloblastin, NP-4, Neutrophil proteinase 4, P29, PR-3, PRTN3, Proteinase 3, Wegener autoantigen

ID gène: 5657

NCBI Accession: NP_002768

UniProt: P24158