Collagen Type I anticorps

N° du produit ABIN111171

Détail du produit anti-Collagen Type I anticorps

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Collagen Type I est non-conjugé

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificité: Both native and heat denatured human collagen type I. No cross reactivity with albumin fractions of human serum or albumins of other animal species. Less than 5 % binding to collagens II and II in ELISA, RIA or Immunoblotting.

Purification: Ammonium sulfate precipitation and chromatography on DEAE-cellulose

Clone: BDI314

Isotype: IgG

Information d'application

Indications d'application: ELISA: Apparent binding constant from titration curves is approximately 0.1 nM. Antibodysuitable for use in sandwich ELISA for specific determination of collagen I, employed aseither a capture antibody or as a second, detecting antibody. Immunohistochemical staining of 10 m thick cryostat sections fixed with acetone orethanol. Staining is similar to that for polyclonal antibodies to interstitial collagen I. Suggested working concentration 10-20 μg/mL. Formaldehyde fixation is notrecommended. Does not work with Western blots. Not suitable for use on paraffin embedded sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL distilled water.

Agent conservateur: Azide free

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Avoid multiple freeze/thaw cycles.

Détails sur Collagen Type I

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Autre désignation: Collagen Type I (COL1 Produits)

Synonymes: anticorps collagen type III alpha 1 chain, anticorps COL3A1

Sujet: Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

ID gène: 1277

NCBI Accession: NP_000079

UniProt: P02452