EpCAM anticorps

N° du produit ABIN111180

Cet anticorps anti-EpCAM est un anticorps Souris Monoclonal détectant EpCAM dans WB, IHC (p) et IHC (fro). Adapté pour Humain.

Aperçu rapide pour EpCAM anticorps (ABIN111180)

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EpCAM est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: HEA125

Détail du produit anti-EpCAM anticorps

Specificité: This antibody recognises the 34 kDa cell surface antigen known as CD326 or Epithelial Cell Adhesion Molecule (Ep-CAM).

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogène: HT29 carcinoma cell line. Spleen cells from immunised BALB/c mice were fused with cells of a mouse myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: Use 5 μL of neat antibody to label 10^6 cells or 100 μL cell suspension. Immunohistochemistry on Frozen Sections: 1/20. Immunohistochemistry on paraffin sections: 1/20. This product requires protein digestionpre-treatment of paraffin sections e. g.0.1 % pronase, 10 minutes. Recommended Positive Control: Human colon and appendix.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.3 containing 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: CD326 / EPCAM / TACSTD1

Sujet: This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against Ep-CAM, many of which have been described as tumour specific molecules on carcinomas. Ep-CAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.Synonyms: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1

ID gène: 4072

NCBI Accession: NP_002345

UniProt: P16422