GFAP anticorps

N° du produit ABIN111187

Cet anticorps Souris Monoclonal détecte spécifiquement GFAP dans WB, IF, IHC (p) et IHC (fro). Il présente une réactivité envers Humain, Rat, Souris et Boeuf (Vache).

Aperçu rapide pour GFAP anticorps (ABIN111187)

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivité: Humain, Rat, Souris, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFAP est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: GF12-24

Détail du produit anti-GFAP anticorps

Specificité: GF 12.24 represents an excellent marker for cell typing. Suitable for prenatal diagnosis of neural tube defects. Polypeptide Reacting: Mr 50 000 glial filament protein GFP. Tumors Specifically Reacting: Astrocytomas, gangliomas, medulloblastomas, mixed gliomas, certain ependymomas, certain teratomas. Tested Reactivities on Cultured Cell Lines: U 333 CG/343MG.

Réactivité croisée (Details): Species reactivity (tested):Human, Bovine, Mouse and Rat.

Purification: Protein A Chromatography

Immunogène: Intermediate filament cytoskeleton from cultured human glioma cells.

Isotype: IgG2a

Information d'application

Indications d'application: Immunoblotting. Immunofluorescence Microscopy. Immunohistochemistry. Cytological MaterialImmunohistochemistry on Frozen Sections and Paraffin-Embedded Sections. Working Dilutions: 1/10 with PBS, pH 7.4, for Immunohistochemical application. Incubation Time: 1 h ar RT, extended with paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1.0 mL distilled water

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Détails sur GFAP

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Autre désignation: GFAP

Sujet: Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytesSynonyms: Glial Fibrillary Acidic Protein

ID gène: 2670

NCBI Accession: NP_001229305

UniProt: P14136