DPP4 anticorps (FITC)

N° du produit ABIN1112104

Détail du produit anti-DPP4 anticorps (FITC)

Antigène: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DPP4 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Attributs du produit: Monoclonal Mouse Anti-Human CD26 FITC is recommended for use in flow cytometry for identification of human receptor for Interleukin-2 (IL-2R) expressing the 55,000 M.W. surface antigen.

Clone: TP1-19

Isotype: IgG2b

Information d'application

Indications d'application: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Commentaires:

Fluorescein isothiocyanate (Molecular Probes).

Prélèvement de l'échantillon: 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD26 FITC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 100 µl of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS ( It better that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Agent conservateur: Sodium azide

Précaution d'utilisation: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Stock: 4 °C

Détails sur DPP4

Antigène: DPP4 (Dipeptidyl-Peptidase 4 (DPP4))

Autre désignation: CD26 (DPP4 Produits)

Synonymes: anticorps ADABP, anticorps ADCP2, anticorps CD26, anticorps DPPIV, anticorps TP103, anticorps MGC81966, anticorps MOP9.8, anticorps MOP9_8, anticorps DPP4, anticorps si:ch73-2d23.3, anticorps Cd26, anticorps Dpp-4, anticorps THAM, anticorps dipeptidyl peptidase 4, anticorps dipeptidyl-peptidase 4 S homeolog, anticorps prolyl oligopeptidase family protein, anticorps dipeptidyl-peptidase 4, anticorps dipeptidylpeptidase 4, anticorps DPP4, anticorps dpp4.S, anticorps AT5G24260, anticorps dpp4, anticorps Dpp4

Sujet: The monoclonal antibody is directed against the CD26- antigen, which is expressed on human activated T- and B- cells. CD26 is required for T cell proliferation. It is expressed on 10-60% of resting T cells in normal peripheral blood. There is a selective decrease of CD4+ CD26+ T cells in HIV-1 infected individuals.

Pathways: Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity