CPA1 anticorps (APC)

N° du produit ABIN1112254

Cet anticorps Souris Monoclonal détecte spécifiquement CPA1 dans IF et FACS. Il présente une réactivité envers Humain et a été mentionné dans 2+ publications.

Aperçu rapide pour CPA1 anticorps (APC) (ABIN1112254)

Antigène: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CPA1 est conjugé à/à la APC

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: CA2

Détail du produit anti-CPA1 anticorps (APC)

Attributs du produit: Monoclonal Mouse Anti-Human Carboxypeptidase A APC for identification of Carboxypeptidase A. CA2 is a mouse monoclonal antibody specific for mast cell carboxypeptidase.

Isotype: IgG

Information d'application

Indications d'application: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Commentaires:

Allophycocyanin (APC), Abs/Em: 651/662 nm.

Prélèvement de l'échantillon: 1. Pipette 50 µl of sample to be analysed (up to 10^6 cells) into each tube. 2. For each sample, add an appropriate volume of conjugated antibody (20 µl) directed to the cell surface antigen of interest and the appropriate isotype control. Incubate for 15 minutes in the dark at room temperature. (This step is only necessary is you want to perform a direct immunofluorescence staining for a cell surface antigen) 3. Add 100 µL of Intracell Reagent A, (Fixative), to each tube. Mix gently. 4. Incubate for 15 minutes at room temperature. 5. Wash once in 2 ml PBS Working Solution 1X. 6. Centrifuge for 5 minutes at 300 xg, then aspirate supernatant, leaving approximately 50 µl of fluid and vortex to ensure that the cell pellet are in suspension. 7. Add 100 µL of Intracell Reagent B (Permeabilization), to each tube. 8. Add the appropriate volume of CPA APC for the intracellular antigen and the appropriate isotype control. 9. Incubate for 15 minutes in the dark at room temperature. 10. Wash once in 2 ml PBS Working Solution 1X. 11. Centrifuge for 5 minutes at 300 xg, then aspirate supernatant, leaving approximately 50 µl of fluid and vortex to ensure that the cell pellet are in suspension. 12. Resuspend the cell pellet in 0,5 ml of 1% paraformaldehyde solution or an appropriate fluid for flow cytometric use, and store in the dark at 2-8 °C. Fixed cells should be analyzed within 24 hours.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing stabilizer solution, PBS 20 mM and 0,09% Sodium azide, pH 7.2.

Agent conservateur: Sodium azide

Précaution d'utilisation: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Stock: 4 °C

Références pour anticorps anti-CPA1 (APC) (ABIN1112254)

Austin, Trowsdale, Rudd, Bodmer, Feldmann, Lamb: "Functional expression of HLA-DP genes transfected into mouse fibroblasts." dans: Nature, Vol. 313, Issue 5997, pp. 61-4, (1985) (PubMed).

Royston, Omary, Trowbridge: "Monoclonal antibodies to a human T-cell antigen and Ia-like antigen in the characterization of lymphoid leukemia." dans: Transplantation proceedings, Vol. 13, Issue 1 Pt 2, pp. 761-6, (1981) (PubMed).

Détails sur CPA1

Antigène: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Autre désignation: CPA