Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

CPA1 anticorps (APC)

CPA1 Reactivité: Humain IF, FACS Hôte: Souris Monoclonal CA2 APC
N° du produit ABIN1112254
  • Antigène Voir toutes CPA1 Anticorps
    CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))
    Reactivité
    • 38
    • 11
    • 7
    • 6
    • 2
    • 2
    • 1
    • 1
    Humain
    Hôte
    • 37
    • 10
    • 1
    Souris
    Clonalité
    • 40
    • 8
    Monoclonal
    Conjugué
    • 31
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CPA1 est conjugé à/à la APC
    Application
    • 44
    • 19
    • 13
    • 13
    • 13
    • 8
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Flow Cytometry (FACS)
    Attributs du produit
    Monoclonal Mouse Anti-Human Carboxypeptidase A APC for identification of Carboxypeptidase A. CA2 is a mouse monoclonal antibody specific for mast cell carboxypeptidase.
    Clone
    CA2
    Isotype
    IgG
    Top Product
    Discover our top product CPA1 Anticorps primaire
  • Indications d'application
    It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
    Commentaires

    Allophycocyanin (APC), Abs/Em: 651/662 nm.

    Prélèvement de l'échantillon
    1. Pipette 50 µl of sample to be analysed (up to 10^6 cells) into each tube. 2. For each sample, add an appropriate volume of conjugated antibody (20 µl) directed to the cell surface antigen of interest and the appropriate isotype control. Incubate for 15 minutes in the dark at room temperature. (This step is only necessary is you want to perform a direct immunofluorescence staining for a cell surface antigen) 3. Add 100 µL of Intracell Reagent A, (Fixative), to each tube. Mix gently. 4. Incubate for 15 minutes at room temperature. 5. Wash once in 2 ml PBS Working Solution 1X. 6. Centrifuge for 5 minutes at 300 xg, then aspirate supernatant, leaving approximately 50 µl of fluid and vortex to ensure that the cell pellet are in suspension. 7. Add 100 µL of Intracell Reagent B (Permeabilization), to each tube. 8. Add the appropriate volume of CPA APC for the intracellular antigen and the appropriate isotype control. 9. Incubate for 15 minutes in the dark at room temperature. 10. Wash once in 2 ml PBS Working Solution 1X. 11. Centrifuge for 5 minutes at 300 xg, then aspirate supernatant, leaving approximately 50 µl of fluid and vortex to ensure that the cell pellet are in suspension. 12. Resuspend the cell pellet in 0,5 ml of 1% paraformaldehyde solution or an appropriate fluid for flow cytometric use, and store in the dark at 2-8 °C. Fixed cells should be analyzed within 24 hours.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The conjugate is provided in liquid form in buffer containing stabilizer solution, PBS 20 mM and 0,09% Sodium azide, pH 7.2.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
    Stock
    4 °C
  • Austin, Trowsdale, Rudd, Bodmer, Feldmann, Lamb: "Functional expression of HLA-DP genes transfected into mouse fibroblasts." dans: Nature, Vol. 313, Issue 5997, pp. 61-4, (1985) (PubMed).

    Royston, Omary, Trowbridge: "Monoclonal antibodies to a human T-cell antigen and Ia-like antigen in the characterization of lymphoid leukemia." dans: Transplantation proceedings, Vol. 13, Issue 1 Pt 2, pp. 761-6, (1981) (PubMed).

  • Antigène
    CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))
    Autre désignation
    CPA (CPA1 Produits)
    Synonymes
    anticorps cpa1, anticorps cbpa1, anticorps CPA, anticorps CPA1, anticorps 0910001L12Rik, anticorps Cpa, anticorps carboxypeptidase A1, anticorps pancreatic carboxypeptidase A1, anticorps Carboxypeptidase A1, anticorps carboxypeptidase A1-like, anticorps carboxypeptidase a1, anticorps carboxypeptidase A1, pancreatic, anticorps CpipJ_CPIJ007169, anticorps CpipJ_CPIJ010802, anticorps CpipJ_CPIJ010805, anticorps MCYG_06789, anticorps MGYG_03840, anticorps cpa1, anticorps cbpa1, anticorps LOC100224866, anticorps CPA1, anticorps Cpa1
Vous êtes ici:
Support technique