ICAM1 anticorps

N° du produit ABIN111278

L’anticorps Souris Monoclonal anti-ICAM1 a été validé pour WB, FACS, IHC (p) et EIA. Il convient pour détecter ICAM1 dans des échantillons de Humain, Rat et Boeuf (Vache). Il y a 8+ publications disponibles.

Aperçu rapide pour ICAM1 anticorps (ABIN111278)

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivité: Humain, Rat, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ICAM1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: MEM-111

Détail du produit anti-ICAM1 anticorps

Purification: Affinity Chromatography on Protein A

Immunogène: Raji Human Burkitt's lymphoma cell line. Genename: ICAM1

Isotype: IgG2a

Information d'application

Indications d'application: Immunohistochemistry on Paraffin Sections: Recommended Dilution: 10 μg/mLPositive tissue: thymus, RE cellsFlow Cytometry. ELISA. Western blotting (Non-Reducing Conditions). Positive Control: RAJI cell line, K562 leukemia cell line, JY cell line, ActivatedT-lymphocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C (addition of a conserving agent is highly recommended) or (in aliquots) at -20 °C.

Références pour anticorps anti-ICAM1 (ABIN111278)

Lee, Sieling, Ochoa, Krutzik, Guo, Hernandez, Rea, Cheng, Colonna, Modlin: "LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 12, pp. 8128-36, (2007) (PubMed).

Andjelkov, Elvenes, Figenschau, Bjorkoy, Knutsen, Seternes, Johansen: "Detection of mRNA transcripts of truncated opiate precursor (POMC) in human cartilage." dans: Cell biochemistry and function, Vol. 24, Issue 3, pp. 229-35, (2006) (PubMed).

Hertel, Lacaille, Strobl, Mellins, Mocarski: "Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus." dans: Journal of virology, Vol. 77, Issue 13, pp. 7563-74, (2003) (PubMed).

Burdick, McCaffery, Kim, Bochner, Konstantopoulos: "Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow." dans: American journal of physiology. Cell physiology, Vol. 284, Issue 4, pp. C977-87, (2003) (PubMed).

Tachimoto, Kikuchi, Hudson, Bickel, Hamilton, Bochner: "Eotaxin-2 alters eosinophil integrin function via mitogen-activated protein kinases." dans: American journal of respiratory cell and molecular biology, Vol. 26, Issue 6, pp. 645-9, (2002) (PubMed).

Bacáková, Mares, Lisá, Svorcík: "Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions." dans: Biomaterials, Vol. 21, Issue 11, pp. 1173-9, (2000) (PubMed).

Bacáková, Mares, Bottone, Pellicciari, Lisá, Svorcík: "Fluorine ion-implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture." dans: Journal of biomedical materials research, Vol. 49, Issue 3, pp. 369-79, (2000) (PubMed).

Nagaraju, Raben, Merritt, Loeffler, Kirk, Plotz: "A variety of cytokines and immunologically relevant surface molecules are expressed by normal human skeletal muscle cells under proinflammatory stimuli." dans: Clinical and experimental immunology, Vol. 113, Issue 3, pp. 407-14, (1998) (PubMed).

Détails sur ICAM1

Antigène: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Autre désignation: CD54 / ICAM1

Sujet: ICAM-1 (MW 90 kD) is a member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.Synonyms: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor

ID gène: 9606

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling