MAP2 anticorps

N° du produit ABIN111291

Détail du produit anti-MAP2 anticorps

Antigène: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poulet, Xenopus laevis, Caille

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MAP2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Thisantibody recognizes (Mr300 kDa) MAP-2 protein from bovine brain. The antibody strongly cross-reacts with MAP-2 from Human, Rat, Mouse, Xenopus, quail and Chicken brains. BM2472 reacts with the High Molecular Weight forms (2a & 2b) of MAP-2 but not with the Low Molecular Weight (70 kDa) form (2c). It reacts with dendrites and cell bodies of neurons.

Purification: Protein G Chromatography.

Immunogène: Bovine brain Microtubule Associated Protein 2.

Clone: AP20

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry on Frozen Sections: 1/500. Immunofluorescence: 1/500. Western Blotting: 1-2 μg/mL for 2hrs at RT. Immunohistology (Formalin/Paraffin): 1-2 μg/mL for 30 min at RT. Staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL (OD280)

Buffer: 0.01 M phosphate buffered saline (PBS) pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Aazide as a preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Références pour anticorps anti-MAP2 (ABIN111291)

Pan, Tibbe, Harms, Reißner, Becker, Dingmann, Mirzaa, Kattentidt-Mouravieva, Shoukier, Aggarwal, Missler, Kutsche, Kreienkamp: "Missense mutations in CASK, coding for the calcium-/calmodulin-dependent serine protein kinase, interfere with neurexin binding and neurexin-induced oligomerization." dans: Journal of neurochemistry, Vol. 157, Issue 4, pp. 1331-1350, (2021) (PubMed).

Détails sur MAP2

Antigène: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Autre désignation: MAP-2 (MAP2 Produits)

Synonymes: anticorps MAP2A, anticorps MAP2B, anticorps MAP2C, anticorps MAP2R, anticorps Mtap2, anticorps MAP-2, anticorps Mtap-2, anticorps xmap2, anticorps MGC53131, anticorps a730034c02, anticorps zgc:103474, anticorps zgc:113974, anticorps microtubule associated protein 2, anticorps microtubule-associated protein 2, anticorps microtubule associated protein 2 S homeolog, anticorps MAP2, anticorps Map2, anticorps map2.S, anticorps map2

Sujet: MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2, two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.Synonyms: MAP2, Microtubule-associated protein 2, Neuronal Marker

ID gène: 4133

NCBI Accession: NP_002365

UniProt: P11137