MAP2 anticorps (Biotin)

N° du produit ABIN111292

Cet anticorps Souris Monoclonal détecte spécifiquement MAP2 dans WB, IF, IHC (p) et IHC (fro). Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Poulet, Caille et Xenopus laevis.

Aperçu rapide pour MAP2 anticorps (Biotin) (ABIN111292)

Antigène: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poulet, Caille, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MAP2 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: AP20

Détail du produit anti-MAP2 anticorps (Biotin)

Specificité: Anti-MAP-2 recognizes (Mr 300 kDa)MAP-2 protein from bovine brain. The antibody strongly cross-reacts with MAP-2 from human, rat, mouse, Xenopus, quail and chicken brains. Anti-MAP-2 (Clone AP20) reacts with the high molecular weight forms (2a & 2b) of MAP-2 but not with the low molecular weight form (2c). Anti-MAP-2 reacts with dendrites and cell bodies of neurons.

Purification: Protein G chromatography

Immunogène: Bovine brain microtubule associated protein 2.

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry on frozen sections: 1/500. Immunofluorescence: 1/500. WesternBlotting: 1-2 μg/mL for 2hrs at RT). Immunohistology (Formalin/paraffin): 1-2 μg/mL for 30 min at RT. [Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mMcitrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. ]Anti-MAP-2 may be used in studies of neuron structure in both normal and malignant braintissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: 0.01 M phosphate buffered saline (PBS) pH 7.2, containing, 1.0 % BSA and 0.09 % sodium azide as a preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.

Détails sur MAP2

Antigène: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Autre désignation: MAP-2

Sujet: Microtubule Associated Protein 2 exists in two high molecular weight forms (2a and 2b) and a low molecular weight form (2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.Synonyms: MAP2, Microtubule-associated protein 2, Neuronal Marker

ID gène: 4133

NCBI Accession: NP_002365

UniProt: P11137