Clathrin Heavy Chain (CLTC) (Heavy Chain) anticorps

N° du produit ABIN111496

Cet anticorps Souris Monoclonal détecte spécifiquement dans WB et IHC (fro). Il présente une réactivité envers Humain, Rat, Boeuf (Vache), Porc et Amphibien.

Aperçu rapide pour Clathrin Heavy Chain (CLTC) (Heavy Chain) anticorps (ABIN111496)

Antigène: Clathrin Heavy Chain (CLTC)

Reactivité: Humain, Rat, Boeuf (Vache), Porc, Amphibien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: CHC5-9

Détail du produit

Épitope: Heavy Chain

Specificité: Clone CHC5.9 represents an excellent marker for detection of receptor mediated endocytosis (for review cf. e.g. Refs. 2 and 3). Polypeptide Reacting: Mr 180 000 polypeptide (clathrin heavy chain) from coated vesicles of various tissues. Structures and Tissues Specifically Detected: Coated vesicles of different organs and tissues (eg brain, mammary gland, ovaries). Reactivities on Cultured Cell Lines (tested so far): HeLa, SV-40, RVF-SMC.

Réactivité croisée (Details): Species reactivity (tested):Human, Bovine, Pig, Rat and Amphibia.

Purification: Gel filtration

Immunogène: Coated vesicles (Clathrin) of Bovine brain.

Isotype: IgM

Information d'application

Indications d'application: Immunohistochemistry on Frozen Tissue: 1/10. (When reconstituted, dilute further with PBS, pH 7.4). Incubation Time: 1 h at RT. Immunoblotting (Western): 1/100-1/500 (ECL). Successful use in Immunoprecipitation was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL distilled water.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détail du antigène

Antigène: Clathrin Heavy Chain (CLTC)

Autre désignation: Clathrin Heavy Chain

Sujet: Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrin is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows Clathrin to form its unique polyhedral network.Synonyms: CLH17, CLTC, CLTCL2, Clathrin heavy chain on chromosome 17, KIAA0034, Membrane Vesicle Marker

ID gène: 281080

NCBI Accession: NP_776448

UniProt: P49951