Clathrin Heavy Chain (CLTC) (Heavy Chain) anticorps

N° du produit ABIN111496

Détail du produit

Antigène: Clathrin Heavy Chain (CLTC)

Épitope: Heavy Chain

Reactivité: Humain, Rat, Boeuf (Vache), Porc, Amphibien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Clone CHC5.9 represents an excellent marker for detection of receptor mediated endocytosis (for review cf. e.g. Refs. 2 and 3). Polypeptide Reacting: Mr 180 000 polypeptide (clathrin heavy chain) from coated vesicles of various tissues. Structures and Tissues Specifically Detected: Coated vesicles of different organs and tissues (eg brain, mammary gland, ovaries). Reactivities on Cultured Cell Lines (tested so far): HeLa, SV-40, RVF-SMC.

Réactivité croisée (Details): Species reactivity (tested):Human, Bovine, Pig, Rat and Amphibia.

Purification: Gel filtration

Immunogène: Coated vesicles (Clathrin) of Bovine brain.

Clone: CHC5-9

Isotype: IgM

Information d'application

Indications d'application: Immunohistochemistry on Frozen Tissue: 1/10. (When reconstituted, dilute further with PBS, pH 7.4). Incubation Time: 1 h at RT. Immunoblotting (Western): 1/100-1/500 (ECL). Successful use in Immunoprecipitation was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL distilled water.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détail du antigène

Antigène: Clathrin Heavy Chain (CLTC)

Autre désignation: Clathrin Heavy Chain (CLTC Produits)

Synonymes: anticorps CHC, anticorps Chc, anticorps CHC17, anticorps CLH-17, anticorps CLTCL2, anticorps Hc, anticorps CLTC, anticorps 3110065L21Rik, anticorps R74732, anticorps F11B9.30, anticorps cb1033, anticorps cltc, anticorps wu:fc03e11, anticorps wu:fc49a11, anticorps wu:fd07f02, anticorps clathrin heavy chain, anticorps Probable clathrin heavy chain 1, anticorps putative clathrin heavy chain, anticorps clathrin heavy chain 1, anticorps clathrin heavy chain Chc1, anticorps clathrin, heavy polypeptide (Hc), anticorps Clathrin, heavy chain, anticorps clathrin, heavy chain a (Hc), anticorps clathrin, heavy chain (Hc) L homeolog, anticorps CHC1, anticorps chc-1, anticorps CLTC, anticorps Chc, anticorps TP03_0480, anticorps LOC5578293, anticorps LMJF_36_1630, anticorps CpipJ_CPIJ014882, anticorps CNJ03270, anticorps ATEG_05434, anticorps CMU_025200, anticorps PMAA_094250, anticorps SJAG_02282, anticorps Smp_154420.1, anticorps PAAG_00730, anticorps MCYG_06136, anticorps VDBG_05048, anticorps MGYG_04658, anticorps cgd8_1270, anticorps Tc00.1047053506167.50, anticorps CHC, anticorps Cltc, anticorps AT3G11130, anticorps cltca, anticorps cltc.L

Sujet: Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrin is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows Clathrin to form its unique polyhedral network.Synonyms: CLH17, CLTC, CLTCL2, Clathrin heavy chain on chromosome 17, KIAA0034, Membrane Vesicle Marker

ID gène: 281080

NCBI Accession: NP_776448

UniProt: P49951