Selectin E/CD62e anticorps (FITC)

N° du produit ABIN111607

Cet anticorps Souris Monoclonal détecte spécifiquement Selectin E/CD62e dans FACS. Il présente une réactivité envers Humain et Porc.

Aperçu rapide pour Selectin E/CD62e anticorps (FITC) (ABIN111607)

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivité: Humain, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Selectin E/CD62e est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Clone: 1-2B6

Détail du produit anti-Selectin E/CD62e anticorps (FITC)

Specificité: This antibody recognizes the CD62E and CD62P cell surface antigens. Although previously thought to recognise only Human CD62E, recent data shows that this antibody also recognizes Human CD62P, binding to a common epitope shared by these members of the selectin family.

Réactivité croisée (Details): Species reactivity (expected):Pig.
Species reactivity (tested):Human.
Also reacts to: CD62P

Purification: Affinity Chromatography on Protein A.

Immunogène: Human E-Selectin (ELAM-1). Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: use 10 μL of neat antibody to label 1 x 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Selectin E/CD62e

Antigène: Selectin E/CD62e (SELE) (Selectin E (SELE))

Autre désignation: CD62E / E-Selectin

Sujet: CD62E is also known as endothelial leukocyte adhesion molecule 1 (ELAM1) or E selectin. It is a type I membrane protein that belongs to the selectin family of surface molecules (along with CD62L and CD62P). Selectins are C type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. CD62E (E selectin) is an endothelial cell specific selectin that is expressed on cytokine induced endothelial cells only after activation with proinflammatory cytokines. In vitro experiments have shown that IL1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of CD62E in an NFkB dependent signalling cascade. CD62E has been associated with blood vessel endothelium in diverse inflammatory situations. The main ligands recognised by CD62E are oligosaccharides related to sialyl lewis x.Synonyms: CD62 antigen-like family member E, ELAM1, Endothelial leukocyte adhesion molecule 1, LECAM2, Leukocyte-endothelial cell adhesion molecule 2, SELE

ID gène: 6401

NCBI Accession: NP_000441

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling