CRH anticorps

N° du produit ABIN111806

L’anticorps anti-CRH Monoclonal Souris est utilisé pour la détection de CRH dans des échantillons de Humain. Il a été validé pour IHC (p) et EIA.

Aperçu rapide pour CRH anticorps (ABIN111806)

Antigène: CRH (Corticotropin Releasing Hormone (CRH))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CRH est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 5D5

Détail du produit anti-CRH anticorps

Specificité: Recognises a different epitope than clone 4H9 (BM357). 5D5 mAb binding decreases only partly after CA treatment. It is possible that the antigen determinant is exposed more openly after DEP modification of histidine residues, whereas this determinant becomes barely accesible after lysine modification. Human serum, containing CRF-binding protein, inhibits the binding of CRF to this antibody.Aff. Const.:1.5 x 10-8M for native CRF, 2 x 10-9M for modified CRF

Purification: Affinity Chromatography on Protein G

Immunogène: Synthetic Human Corticotropin Releasing Factor

Isotype: IgG1

Information d'application

Indications d'application: ELISA: High binding constant allows this antibody to be used in a sandwich ELISA withclone number 4H9 (BM357), Sensitivity: 25 pg/mL. Immunohistochemistry on frozen sections: A 4 μg/mL solution has worked well on paraffinsections, after microwave pre-treatment.0.1 % trypsin digestion may also be necessary,The detection system used was avidin-biotin. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CRH

Antigène: CRH (Corticotropin Releasing Hormone (CRH))

Autre désignation: Corticoliberin

Sujet: CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).Synonyms: CRF, CRH, Corticotropin-Releasing Factor, Corticotropin-Releasing Hormone

ID gène: 1392

NCBI Accession: NP_000747

UniProt: P06850

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour