CD163 anticorps

N° du produit ABIN111834

Cet anticorps anti-CD163 est un anticorps Souris Monoclonal détectant CD163 dans WB, FACS, IP, IF, IHC (p) et IHC (fro). Adapté pour Rat.

Aperçu rapide pour CD163 anticorps (ABIN111834)

Antigène: CD163

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD163 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ED2

Détail du produit anti-CD163 anticorps

Specificité: This antibody recognises the Rat CD163 cell surface glycoprotein, a 175 kDa molecule also known as ED2. It does not react with Human.

Réactivité croisée (Details): Species reactivity (tested):Rat.

Purification: Affinity Chromatography on Protein A

Immunogène: Rat Spleen cell homogenate. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0Ag 14 mouse myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Western blot. Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/10-1/100 diluted CD163 antibody to label 10^6 cells in 100μL. Immunohistochemistry on Frozen Sections: 1/50-1/100Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval usingpronase prior to staining of paraffin sections. IHC on paraffin-embedded material following PLP fixation(Periodate-lysine-paraformaldehyde) - refer to Whiteland et al (Ref.5. )Histology Positive Control Tissue: liver.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CD163

Antigène: CD163

Autre désignation: CD163

Sujet: CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163 is expressed by approximately 50 % of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130

ID gène: 9332

NCBI Accession: NP_004235

UniProt: Q86VB7