SFTPD anticorps (Lectin Domain) (Biotin)

N° du produit ABIN111842

L’anticorps Souris Monoclonal anti-SFTPD a été validé pour WB, IHC (fro) et EIA. Il convient pour détecter SFTPD dans des échantillons de Humain et Rat.

Aperçu rapide pour SFTPD anticorps (Lectin Domain) (Biotin) (ABIN111842)

Antigène: SFTPD (Surfactant Protein D (SFTPD))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SFTPD est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: VIF11

Détail du produit anti-SFTPD anticorps (Biotin)

Épitope: Lectin Domain

Specificité: Antigen Distribution on Tissue Sections: alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on Rat skin, colon, kidney and liver.

Purification: Affinity Chromatography

Immunogène: Purified rat surfactant protein D from bronchoalveolar lavage. Remarks: The antigen epitope is localized in the Lectin domain of SP-D.

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry (IHC) on Frozen sections (2 μg/mL (1/200) for rat cryosections) andParaffin sections. ELISA. Western Blots. Suggested positive control: Rat lung.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2, 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Détails sur SFTPD

Antigène: SFTPD (Surfactant Protein D (SFTPD))

Autre désignation: SFTPD

Sujet: Surfactant protein D (SP-D) is a Ca2+-dependent carbohydrate-binding protein and is structurally similar to other C-type mammalian lectins, such as conglutinin and SP-A. It has a molecular size of approximately 43 kDa in its reduced state, 620 kDa in non-dissociating conditions. SP-D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.Synonyms: COLEC7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, Pulmonary surfactant-associated protein D, SFTP4, SP-D

ID gène: 25350

NCBI Accession: NP_037010

UniProt: P35248