CD209b Antigen (CD209B) anticorps

N° du produit ABIN111861

Détail du produit

Antigène: CD209b Antigen (CD209B)

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: ER-TR9 antibody is a very useful marker for the identification of macrophage subpopulations present in the splenic marginal zone, lymph node medulla and other organs. In combination with MOMA-1 (Cat-Nr SM066P), the murine metallophilic macrophage marker, a detailed characterization of murine splenic marginal zone macrophages is obtained. ER-TR9 is also useful when studying phagocytosis of neutral polysaccharides since the antibody selectively inhibits uptake of these glycans by macrophages. The antigen recognized by ER-TR9 has recently been shown to be the Murine analogue of the Human DC-SIGN (Dendritic Cell - Specific ICAM-3 Grabbing Non Integrin), named SIGN-R1. Antigen Distribution Antigen Distribution on Isolated cells: The antigen is found on a subpopulation of phagocytic macrophages isolated from the spleen and showing acid phosphatase and moderate non-specific esterase activity. These phagocytes selectively ingest neutral polysaccharides such as Ficoll. Antigen Distribution on Tissue Sections: Subpopulation of resident macrophages in the splenic marginal zone which are in the proximity of a certain B cell subpopulation (μ+, d-). It is also found on a subpopulation of macrophages localized in the medullary sinuses and trabecular sinuses of lymph nodes. Furthermore, macrophage subpopulations in other organs, such as some connective tissue macrophages in the dermis, may also show ER-TR9 antigen expression. This antibody is specific for a Mouse subpopulation of mature tissue macrophages present in the splenic marginal zone, lymph node medullary and trabecular sinuses.

Réactivité croisée (Details): Species reactivity (tested):Mouse: Subpopulation of mature tissue macrophages present in the splenic marginal zone, lymph node medullary and trabecular sinuses.

Purification: Affinity Chromatography

Immunogène: Thymus cells. Remarks: The antigen is a Glutaraldehyde (0.05%) resistant protein expressed in the cytoplasm and on the cell surface.

Clone: ER-TR9

Isotype: IgM

Information d'application

Indications d'application: Immunohistchemistry on Frozen Sections: 0.5 μg/mL (1/600). Does not react on routinely processed Paraffin sections. Recommended Positive Control: Mouse spleen. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.3 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 0.1 % Kathon as a preservative

Agent conservateur: Kathon CG

Précaution d'utilisation: This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Référence

Weisel, Appelt, Schneider, Horlitz, van Rooijen, Korner, Mach, Winkler: "Unique requirements for reactivation of virus-specific memory B lymphocytes." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 7, pp. 4011-21, (2010) (PubMed).

Détail du antigène

Antigène: CD209b Antigen (CD209B)

Autre désignation: CD209b / DC-SIGNR1 (CD209B Produits)

Synonymes: anticorps 1810030I22Rik, anticorps OtB7, anticorps SIGNR1, anticorps mSIGNR1, anticorps CD209b antigen, anticorps Cd209b

Sujet: Synonyms: CD209 antigen-like protein B, DC-SIGN-related protein 1, OtB7

ID gène: 69165

NCBI Accession: NP_001032889

UniProt: Q8CJ91