Granulocytes anticorps (Biotin)

N° du produit ABIN111864

Détail du produit

Antigène: Granulocytes

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Affinity Chromatography

Immunogène: Peritoneal cells.

Clone: RK-4

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: 1/50-1/100. Immunohistochemistry on frozen sections: 0.75 μg/mL (1/400),does not react on routinely processed paraffin sections. Suggested positive control: Rat spleen, blood smears. Antigen Distribution on Isolated Cells: Only a low percentage of cells from peripheralblood stains positive. Positive on peritoneal cells 18h after thioglycollate injection (95 % granulocytes). Negative on peritoneal cells 4 days after thioglycollate injection. Negative on isolated lymphocytes, monocytes,erythrocytes and platelets. See Table 1. for details.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.3 mg/mL IgG

Buffer: Stock Solution contains PBS, pH 7.2 with 0.01 % Thimerosal as preservative and 10 mg/mL BSA as stabilizer

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Détail du antigène

Antigène: Granulocytes

Sujet: Granulocytes are a type of white blood cell filled with microscopic granules that are little sacs containing enzymes, compounds that digest microorganisms. They are part of the innate immune system and have somewhat nonspecific, broad-based activity. They do not respond exclusively to specific antigens, as do B-cells and T-cells. Neutrophils, eosinophils and basophils are all types of granulocytes. They are named by the staining features of their granules in the laboratory: Neutrophils have "neutral" subtle granules, Eosinophils have prominent granules that stain readily with the acid dye eosin, and Basophils have prominent granules that stain readily basic (non acidic) dyes.Synonyms: Granulocyte cells