CD163 anticorps

N° du produit ABIN111878

L’anticorps Souris Monoclonal anti-CD163 a été validé pour WB, FACS et IHC (fro). Il convient pour détecter CD163 dans des échantillons de Humain et Singe.

Aperçu rapide pour CD163 anticorps (ABIN111878)

Antigène: CD163

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD163 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RM3-1

Détail du produit anti-CD163 anticorps

Specificité: This antibody is specific for CD163. Clone RM 3/1 recognizes a membrane glycoprotein on monocytes and macrophages which is expressed in intermediate and late inflammatory stages. Does not react with lymphocytes, granulocytes or platelets. Positive staining can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar macrophages, a major population of macrophages in the placenta, varying degrees of macrophages in inflamed tissues, including tumorous tissue depending on the inflammatory stage. Red pulp, but not white pulp macrophages of the spleen, and cortical macrophages of the thymus are detected. Macrophages in the synovialis of patients with rheumatoid arthritis. In alveolar macrophages and in Kupffer cells a double staining can be observed with clone 25F9 which is not the case in other tissues.

Réactivité croisée (Details): Species reactivity (tested):Human, Monkey. Negative in Pig, Rabbit and Guinea Pig.

Purification: Affinity Chromatography

Immunogène: Human monocytes, particularly after dexamethasone treatment or after 2-5 days in culture

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry on Frozen Sections: 4 μg/mL (1/100). Does not work in routinely processed paraffin sections. Has been described to work in FACS and Western blots. Recommended Positive control: Human placenta.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water to a concentration of 0.4 mg/mL.

Concentration: 0.4 mg/mL

Buffer: Stock solutions contains PBS, pH 7.2, 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: This product is photosensitive and should be protected from light

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted Prior to and after reconstitution at 2-8 °C. DO NOT FREEZE!

Détails sur CD163

Antigène: CD163

Autre désignation: CD163

Sujet: CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163-positive cells include skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and some thymus macrophages. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130

ID gène: 9332

NCBI Accession: NP_004235

UniProt: Q86VB7