CD93 anticorps

N° du produit ABIN111895

Cet anticorps Souris Monoclonal détecte spécifiquement CD93 dans FACS et IHC (fro). Il présente une réactivité envers Humain.

Aperçu rapide pour CD93 anticorps (ABIN111895)

Antigène: CD93

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD93 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: X-2

Détail du produit anti-CD93 anticorps

Specificité: Monoclonal antibody X-2 recognizes a 110 kDa O-sialoglycoprotein on granulocytes, monocytes and endothelial cells. Upon differentiation of cultivated monocytes into macrophages, CDw93 ceases to be expressed on day 5-6, which makes it a very useful marker to differentiate those two cell populations. Antigen distribution on isolated cells: Positive with all granulocytes, with staining increasing transiently upon maturation. Antigen expression is increased by TNFα, GM-CSF, (fMLP) treatment. No effect on superoxide production by binding to neutrophils has been noted. PMA stimulation causes down regulation of the antigen. Positive with monocytes following fMLP, TNF, GM-CSF treatment. Antigen expression decreases on cultured monocytes. Positive on AML cases with monocytic component. Positive on cell lines HL-60, Namalva, K562, THP-1, U937, negative on HMC-1 (human mast cell line). Antigen distribution on tissue sections: Monoclonal antibody X-2 stains epitheloid cells and Rosai-Dorfman cells and most endothelial cells in the synovial membrane of RA patients.

Purification: Affinity chromatography.

Immunogène: Human monocytes

Isotype: IgG1

Information d'application

Indications d'application: Tested for immunohistochemistry (IHC, Frozen sections: 2 μg/mL (1: 100), Does not react onroutinely processed paraffin sections. Suggested positive control: Human tonsil. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.2 mg/mL IgG

Buffer: PBS, pH 7.2 with 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.01 % Thimerosal as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody at -20 °C. Ship at 2-8 °C.

Détails sur CD93

Antigène: CD93

Autre désignation: CD93 / C1QR1

Sujet: CD93 a cell-surface glycoprotein and type I membrane protein involve in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.Synonyms: C1q receptor, C1q/MBL/SPA receptor, CDw93, Complement component 1 q subcomponent receptor 1, Complement component C1q receptor, MBL receptor, MXRA4, Matrix-remodeling-associated protein 4, SPA receptor

ID gène: 22918

NCBI Accession: NP_036204

UniProt: Q9NPY3